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| Registros recuperados: 16 | |
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| Claus,Marlise Pompeo; Lunardi,Michele; Alfieri,Amauri Alcindo; Otonel,Rodrigo Alejandro Arellano; Ferracin,Lara Munique; Fungaro,Maria Helena Pelegrinelli; Alfieri,Alice Fernandes. |
| The common occurrence of multiple papillomavirus infections has been shown in several studies involving the human host. However, investigations with the aim of identifying mixed papillomavirus infections in cattle have been conducted only recently. In the current work we describe a co-infection with two different bovine papillomavirus (BPV) types that was identified in a bovine teat papilloma. The skin wart was obtained from a cow belonging to a Brazilian beef herd. A PCR assay was carried out with the FAP primer pair, which amplifies a partial segment of the L1 gene (approximately 478 bp), and the amplicon was submitted to direct sequencing. Because nucleotide sequences with satisfactory quality scores were not obtained, the amplicon was cloned and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Cutaneous papillomatosis; BPV; Co-infection. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700012 |
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| Takiuchi,Elisabete; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Filippsen,Patrícia; Alfieri,Amauri Alcindo. |
| Bovine coronavirus (BCoV) is a known cause of winter dysentery (WD) in adult cattle. The morbidity of the disease is high, that results in a significant decrease in milk production and consequently, economic losses. In the present study, we report on a classical outbreak of WD that affected a high-production Holstein dairy herd raised in a tropical country. The lactating batch included 154 cows, and 138 (90%) presented diarrhea in a short (nine days) period of time. Three (2%) cows died. The other batches of animals did not become ill. The evolution of the disease in the herd, including the clinical signs and epidemiological features, strongly suggested a WD case. Semi-nested PCR and RFLP confirmed that BCoV was the cause of the infection. Samples tested... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Dairy herd; Winter dysentery; Diarrhea; Bovine coronavirus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700008 |
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| Silva,Luis César da; Takiuchi,Elisabete; Médici,Kerlei Cristina; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo. |
| A resposta imunológica humoral de bovinos vacinados com o vírus da diarréia viral bovina (BVDV) inativado, tendo como adjuvante o cloreto de dimetildioctadecilamônio (DDA cloreto) associado ao hidróxido de alumínio (vacina B), foi comparada com uma vacina contendo o mesmo antígeno adsorvido apenas com hidróxido de alumínio (vacina A). Duas semanas após a segunda dose foi avaliado o título de anticorpos neutralizantes dos animais que receberam as duas preparações de antígenos. Os animais que receberam a vacina B apresentaram melhor resposta imune humoral quando comparados com os animais vacinados com a vacina A. O título médio de anticorpos neutralizantes, expresso em Log2, dos animais que receberam a vacina B foi superior (P<0,05) ao observado no grupo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Vírus da diarréia viral bovina; Cloreto de dimetildioctadecilamônio; Vacina; Adjuvante. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300009 |
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| Linares,Rita de Cássia; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Médici,Kerlei Cristina; Feronato,César; Grieder,Wladimir; Alfieri,Amauri Alcindo. |
| Neonatal diarrhea is one of the main causes of morbidity and mortality in piglets, and it leads to significant economic losses for pig farmers worldwide. The aim of this study was to determine the frequency of diagnosis, age group, and association of group A rotavirus (GARV) infection with diarrhea in piglets from pig herds in two (south and center-west)Brazilian geographical regions. The frequency of GARV diagnosis was evaluated between 2004 and 2007, using SS-PAGE on 681 fecal samples (428 diarrheic and 253 with normal consistency) from 1-4 week-old piglets. The animals were selected from 130 pig herds and 80 counties in the states of Rio Grande do Sul, Santa Catarina, Paraná, Mato Grosso do Sul, and Mato Grosso, Brazil. None of the herds were vaccinated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Piglet; Neonatal diarrhea; Group A rotavirus; SS-PAGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700009 |
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| Headley,Selwyn Arlington; Bodnar,Lívia; Fritzen,Juliana T.T.; Bronkhorst,Dalton Evert; Alfieri,Alice Fernandes; Okano,Werner; Alfieri,Amauri Alcindo. |
| Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brainstem dysfunctions; Rhombencephalitis; Neuropathology; Listeriolysin gene; Epidemiology; Listeria monocytogenes. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300036 |
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| Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo. |
| From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008 |
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| Lunardi,Michele; Claus,Marlise Pompeo; Lisbôa,Júlio Augusto Naylor; Amude,Alexandre Mendes; Headley,Selwyn Arlington; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo. |
| Bovine herpesvirus 5 is a DNA virus that has been associated with meningoencephalitis in young cattle. While its clinical diagnosis is obscured by other major diseases that also produce similar neurological disease in cattle, the use of conventional virological techniques is hampered by the establishment of a lifelong latent infection in the host and the difficulty in differentiating BoHV-1 and BoHV-5. The aim of the current report is to describe the clinical and epidemiological aspects observed in a natural outbreak of BoHV-5 meningoencephalitis in a dairy cattle herd from Brazil. In the outbreak, the affected animals consisted of nine calves, which presented three possible forms of the neurological disease, subjectively classified as peracute, acute, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Central nervous system; Bovine herpesvirus 5; Multiplex-PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700011 |
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| Médici,Kerlei Cristina; Alfieri,Amauri Alcindo; Alfieri,Alice Fernandes. |
| A detecção de anticorpos anti-Herpesvírus Bovino tipo 1 (BHV-1) foi realizada, através da técnica de soroneutralização, em 1235 amostras de soro de bovinos adultos, não-vacinados contra Rinotraqueíte Infecciosa Bovina. As amostras de soro analisadas foram colhidas em 81 rebanhos, com histórico de problemas reprodutivos, incluindo animais com aptidão para carne e leite, provenientes de 30 municípios do Estado do Paraná. Na amostragem proveniente de rebanhos leiteiros, 41,9% (409/977) das amostras de soro e 90,5% (57/63) dos rebanhos foram considerados positivos. Em bovinos de corte, o índice de soropositividade foi de 50,8% (131/258) e 100% (18/18) para amostras de soro e rebanhos, respectivamente. As freqüências de 43,7% (540/1235) de animais e 92,6%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovinos; Herpesvirus bovino tipo 1; Sorologia; Soroneutralização; Problemas reprodutivos. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200025 |
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| Registros recuperados: 16 | |
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